1072 Neurotropin reduces dry skin induced alloknesis via the descending inhibitory system

Alloknesis, itch hypersensitivity to normally innocuous mechanical stimuli, has been reported in various mouse models and patients including xerosis atopic dermatitis (AD). We previously that repeated administration of Neurotropin (NTP), an analgesic agent widely used Japan China, attenuates scratching behavior a model AD. NTP also exert effect via the descending inhibitory system, intrinsic system from brain spinal cord consisting noradrenergic serotonergic pathways. Here, we investigated on alloknesis clarify involvement dry skin pruritus produced by daily cutaneous application shaved rostral back acetone-ether (1:1) mixture then water (AEW) for 7 days. Alloknesis was scored as number scratches response stimulation von Frey filaments 30 times treated area. A single intraperitoneal suppressed score whereas H1-receptor antagonist bepotastine had no effect. performed intrathecal Yohimbine, selective alpha 2-adenoceptor antagonist, or Methysergide malate, mixed 5-HT1 5-HT2 receptors AEW-treated mice before NTP, scores were quantified. The cancelled these antagonists, which suggested therapeutic skin-induced activating both